| Date | Home | Away | Result |
|---|---|---|---|
| 8/22/25 1:30 PM | Pescara Calcio | FC Cesena | 1-3 |
| 8/23/25 12:00 PM | Virtus Entella | Juve Stabia | 1-1 |
| 8/23/25 12:00 PM | FC Empoli | Padova Calcio | 3-1 |
| 8/23/25 2:00 PM | AC Monza | Mantova 1911 | 1-0 |
| 8/23/25 2:00 PM | Palermo FC | AC Reggiana 1919 | 2-1 |
| 8/24/25 12:00 PM | Spezia Calcio | Carrarese Calcio 1908 | 0-2 |
| 8/24/25 12:00 PM | FC Venice | SSC Bari | 2-1 |
| 8/24/25 2:00 PM | Frosinone Calcio | US Avellino | 2-0 |
| 8/24/25 2:00 PM | US Catanzaro 1929 | FC Südtirol | 1-1 |
| 8/25/25 1:30 PM | Sampdoria Genua | Modena FC | 0-2 |